Composite

Part:BBa_K3396011:Design

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-25)


GFP-Fluc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 843
    Illegal NgoMIV site found at 2187
    Illegal NgoMIV site found at 2208
    Illegal AgeI site found at 1911
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644
    Illegal SapI.rc site found at 2093


Design Notes

We bind the GFP with Fluc to avoid the mistake conclusion caused by unteady expression of the plasmid.

Source

GFP was derived from the reporter device used by Elowitz's repressilator Elowitz. Elowitz obtained the GFP tagged sequence (gfpmut3*) published by Andersen and coworkers Andersen to build the reporter device. Fluc was obtained from PCR.

References